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Yes, you absolutely can! You will have to us the EthereumJS-tx package from NPM, you can get more information here: https://github.com/ethereumjs/ethereumjs-tx. It's as simple as:
const EthereumTx = require('ethereumjs-tx').Transaction
const privateKey = Buffer.from(
'e331b6d69882b4cb4ea581d88e0b604039a3de5967688d3dcffdd2270c0fd109',
'hex',
)
const ...
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